Search results for "genetics [Insulins]"
showing 8 items of 8 documents
LPS-mediated production of pro/anti-inflammatory cytokines and eicosanoids in whole blood samples: Biological effects of +896A/G TLR4 polymorphism in…
2011
Toll-like receptors (TLRs) are the principal mediators of rapid microbial recognition: the lipopolysaccharide (LPS) receptor TLR4 seems to have a paradigmatic role. Single nucleotide polymorphisms (SNPs) in the TLR4 gene, such as +896A/G, known to attenuate receptor signaling, have been described. The +896A/G SNP is significantly less frequent in patients with myocardial infarction, Alzheimer's disease or prostate cancer, whereas it is overrepresented in centenarians. To clarify and confirm the biological effects of +896A/G SNP and its role in the pathophysiology of age-related diseases and longevity, we assessed the levels of IL-6, TNF-α, IL-10 and eicosanoids (LTB4 and PGE2) in LPS-stimul…
X Chromosome Contribution to the Genetic Architecture of Primary Biliary Cholangitis
2021
Background & aims: Genome-wide association studies in primary biliary cholangitis (PBC) have failed to find X chromosome (chrX) variants associated with the disease. Here, we specifically explore the chrX contribution to PBC, a sexually dimorphic complex autoimmune disease. Methods: We performed a chrX-wide association study, including genotype data from 5 genome-wide association studies (from Italy, United Kingdom, Canada, China, and Japan; 5244 case patients and 11,875 control individuals). Results: Single-marker association analyses found approximately 100 loci displaying P < 5 × 10-4, with the most significant being a signal within the OTUD5 gene (rs3027490; P = 4.80 × 10-6; odds…
Supplementary figure: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen
2020
Supplementary figure 1 – Applicability of LN2 method to break ddPCR emulsion. ddPCR reactions were prepared with ddPCR™ Supermix for Probes (no dUTP) and Droplet Generation Oil for Probes (three tubes on the left) or ddPCR™ Supermix for EvaGreen® and Droplet Generation Oil for EvaGreen® (three tubes on the right). The tubes (A) and (D) show the emulsion before using LN2 method, and tubes (B), (C), (E) and (F) shows the oil and water layers after breaking the emulsion.
Supplementary table: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen
2020
Supplementary table - Raw data from experiments studying DNA recovery from droplets using chemical and physical procedures.
data for: Northern European Salmo trutta (L.) populations are genetically divergent across geographical regions and environmental gradients.
2019
SNP genotype data used in: Bekkevold, D. , Höjesjö, J. , Nielsen, E. E., Aldvén, D. , Als, T. D., Sodeland, M. , Kent, M. P., Lien, S. and Hansen, M. M. (2019), Northern European Salmo trutta (L.) populations are genetically divergent across geographical regions and environmental gradients. Evol Appl. doi:10.1111/eva.12877.The file is a comma-separated text file, where each row gives a list of 3782 genotypes; one row per individual (2090 total). Each individual is denoted by its collection ID, given as a 'River number' following Table 1 in Bekkevold et al. (doi:10.1111/eva.12877), followed by an underscore (_), followed by 'Individual number'. Individual SNP locus names are given in the top…
Supplemental Table 1. Spearman rank order correlations between individual analysis methods. Significant (p<0.05) coefficients (rs) are indicated i…
2019
Supplemental Table 1. Spearman rank order correlations between individual analysis methods. Significant (p<0.05) coefficients (rs) are indicated in bold. Supplemental Table 2. Impact of amount of starting DNA on telomere lengths (n=3) calculated using 4 different analysis methods and applying 2 different equations.
Supplemental Figure 1 Southern blot images of telomere profiles for (A) 15 human cell lines and (B) 3 peripheral blood samples. A total of 2.0 g of …
2022
Supplemental Figure 1 Southern blot images of telomere profiles for (A) 15 human cell lines and (B) 3 peripheral blood samples. A total of 2.0 g of DNA was loaded for each cell line in (A), and 1.0, 1.5, 2.0 and 2.5 g of DNA, as indicated above each lane, for peripheral blood (PB) samples in (B). bp=base pairs
Demographic, Genealogical and morphological characterization of Pantelleria donkey
2004
A demographic, genetic and morphological evaluation on Pantelleria donkey's population was carried out to give a contribution for the exploitation of this old donkey breed. Genealogical data were analysed by the software Minbreed v 1.0. Pedigree is composed by 4 generations. The inbreeding of the population (Fwhole population = 0.4 %) resulted very low than the expectations; therefore, the inbreeding coefficients resulted low in the males F male, = 0.9 % and in the females Ffemale = 0.0 %. On the other hand, the mean relationship among the 378 couplings resulted very high a1j = 15,1 %. The morphological study was carried out on 20 subjects. For each animal the most important morphologic…